hskm cells  (PromoCell)

Journal: Heliyon

Article Title: Fatty acid composition and anti-cancer activity of essential oil from Tenebrio molitor larvae in combination with zoledronic acid on prostate cancer

doi: 10.1016/j.heliyon.2024.e40012

Figure Lengend Snippet: Effect of EOTM and zoledronic acid on hSKM cell line A control (No Treat), B: Cells were treated with 20 μM zoledronic acid, C: cells were treated with 1 % EOTM, D: Cells were treated with 20 μM zoledronic acid in combination with 1 % EOTM.

Article Snippet: The LNCaP and PC3 prostate cell lines and the hSKM fibroblast cell line got from Pasteur Institute of Iran.

Techniques: Control

Journal: Neoplasia (New York, N.Y.)

Article Title: Amelioration of muscle wasting by gintonin in cancer cachexia

doi: 10.1016/j.neo.2021.11.008

Figure Lengend Snippet: GT protects against the atrophy of primary normal Human Skeletal Myoblasts (HSkM). (A–C) HSkM myoblast were differentiated to myotube for 7 days in differentiation media. Differentiated cells were treated with different concentrations of GT in the presence or absence of TNFα (10 ng/mL) for 3 days and then stained with anti-MHC Ab. (A) Representative images of myotube cultures were captured with a phase-contrast microscope (100x magnification). (B) Average myotube diameters and (C) the number of nuclei per myotube were quantified from more than 100 myotubes in 10 randomly chosen fields of each condition using ImageJ software. The data were shown as mean ± SEM (* P ≤ 0.05; *** P ≤ 0.0001). (D, E) HSkM myotubes were treated with GT (100 ng/mL) together with TNFα (10 ng/mL) for 8 h. Cells then were isolated, and the protein levels of Atrogin-1 and MuRF-1 were evaluated by western blot. (D) Representative images were shown. (E) Images were measured by ImageJ software. The data were shown as mean ± SEM ( n =3; * P ≤ 0.05).

Article Snippet: The Human skeletal myoblast (HSkM) cells were obtained from Thermo Fisher Scientific.

Techniques: Staining, Microscopy, Software, Isolation, Western Blot